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Mutagenesis for ACMV resistance in a Ghanian cassava cultivar ‘Bosom nsia’

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by Ahiabu, R.K.; Lokko, Y.; Danso, K.; Klu, G.Y.P. (Biotechnology and Nuclear Agricultural Research Inst., Ghana Atomic Energy Commission, Legon (Ghana))

Breeding for resistance to the African Cassava Mosaic Virus (ACMV) disease in the Ghanian cassava cultivar ‘Bosom nsia’ has been on-going for the past four years at the Biotechnology and Nuclear Agricultural Research Institute. Protocols for in vitro culture from shoot meristems and acclimation of plantlets were established. Radiosensitivity tests on the regenerated plantlets indicated LD50 of 40 Gy, and doses of 25, 30 and 35 Gy were suitable for mutagenesis. These doses were applied to in vivo and in vitro grown plants, and selection was carried out in three propagations. Four variants, selected under field conditions with high viral incidence, were analyzed for virus particles with three virus indexing techniques. Polymerase chain reaction (PCR) product analysis of DNA extracts from Nicotiana benthamiana test plants, inoculated with sap from the leaves of variants, confirmed the presence of virus particles in all variants. Inoculation and ELISA tests suggested ACMV tolerance in selected variants. (author). 24 refs, 3 figs, 1 tab

Source/Report: Joint FAO/IAEA Div. of Nuclear Techniques in Food and Agriculture, Vienna (Austria); 145 p; ISSN 1011-4289; Worldcat; Jul 1997; p. 9-18; Final research co-ordination meeting on improvement of basic food crops in Africa through plant breeding, including the use of induced mutations; Naples (Italy); 30 Oct – 3 Nov 1995; IAEA-TECDOC–951; CONTRACT IAEA-R-5415/R3/IG

Publ. Year: 1997

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